How cell maturation is regulated by circadian and metabolic rhythms

Summary

Research program to mechanistically understand how circadian and metabolic rhythms regulate cell maturation and maintain cell maturity, with implications for regenerative medicine and disease treatment.

What they want

The research program will focus on two areas: 1) How do circadian and metabolic rhythms determine cell maturation? 2) How do circadian and metabolic rhythms maintain cell maturity? These questions will be addressed in defined human in vitro and mouse in vivo model systems. Projects include: a) using in situ multimodal mapping of single-cell gene expression and functional states to determine how circadian clock transcription factors program, synchronize, and entrain maturing cellular activities; b) using in vivo multiplexed gene editing to elucidate molecular mechanisms by which clock components and feeding sustain mature tissue chronophysiology.

Technical requirements

in situ multimodal mapping of single-cell gene expression and functional states
in vivo multiplexed gene editing