Summary
This research project aims to identify global rejuvenation mechanisms in planarian tissues by spatially profiling microenvironmental niches and RNA granules in young, old, and regenerated tissues, using a high-resolution multi-Omic method called Ex-Scope.
What they want
The project involves characterizing RNA granules and soluble transcriptomes in planarian stem cells and oocytes, hypothesizing distinct transcriptome contents in granular structures. Additionally, it proposes to profile microenvironmental niches and their changes during aging and rejuvenation, hypothesizing that aging and rejuvenation affect cellular, tissue-level, and subcellular transcriptome structures, which are crucial for tissue function and homeostasis.
Deliverables
- Characterization of RNA granules and soluble transcriptomes in planarian stem cells and oocytes
- Profiling of microenvironmental niches and their changes during aging and rejuvenation
Technical requirements
- Ex-Scope technology (integrates Expansion Microscopy and Seq-Scope)
- Submicrometer-resolution ST (spatial transcriptomic) technology
- mRNA single-cell data on young, old, and regenerated planarians as a reference dataset
