Summary
This project aims to develop a 3D bioprinting strategy using cell-only bioinks to create prevascularized osteochondral tissue constructs for regenerating damaged knee tissue.
Individual cell bioprinting to generate multi-tissue type condensations for osteochondral tissue regeneration
US · IL
NIH
grant
awarded
#nih-5R01AR081448-04
What they want
The work involves developing a cellular condensation strategy without scaffolds, using 3D bioprinting to directly assemble prevascularized osteochondral tissue constructs from human stem cells and endothelial cells. This will be done within a photocurable liquid-like solid, shear-thinning, and rapid self-healing microgel slurry, with spatially controlled presentation of tissue-specific growth factors. Microgel photocrosslinking will provide temporary mechanical stability. The strategy will print seamlessly continuous two-phase osteochondral tissue constructs with a prevascularized bone phase and a cartilage phase.
Deliverables
- Determine the role of microgel properties on the resolution and fidelity of cell-only 3D printed constructs
- Engineer prevascularized osteochondral constructs with individual cell-only bioinks by spatiotemporally controlled delivery of vasculogenic, osteogenic, and chondrogenic growth factors
- Determine the clinical potential of 3D printed prevascularized osteochondral constructs by evaluating new osteochondral tissue formation and integration with host vascular networks and bone and cartilage repair in a full-thickness osteochondral rabbit defect model
Technical requirements
- 3D bioprinting
- Cell-only bioink
- Photocurable liquid-like solid, shear-thinning and rapid self-healing microgel slurry
- Microgel photocrosslinking
- Human stem cells
- Endothelial cells
- Vasculogenic, osteogenic, and chondrogenic growth factors
- Full-thickness osteochondral rabbit defect model
