Summary
This project aims to define the role of the monocarboxylate transporter slcf-1 in Caenorhabditis elegans intestinal calcium dynamics and metabolism, with implications for human health and disease.
Determine the role of a monocarboxylate transporter in metabolism and intestinal motility
US · IL
NIH
grant
awarded
#nih-1F31DK145217-01A1
What they want
The overarching goal is to define the role of slcf-1 in intestinal Ca2+ dynamics and metabolism. Aim 1 will analyze how mutations in slcf-1 affect Ca2+ waves by quantifying intestinal calcium wave (ICW) properties, performing tissue-specific rescue, and examining SLCF-1::GFP localization. Aim 2 will determine SLCF-1 substrates and how SLCF-1 is dynamically regulated using HEK293T transport assays and mutant slcf-1 promotor constructs. Aim 3 will investigate the metabolic consequences of slcf-1 loss, assessing mitochondrial morphology, activity, and stress, alongside broader metabolic pathway alterations.
Deliverables
- Quantification of intestinal calcium wave (ICW) properties (intervals, rise/fall times)
- Results from tissue-specific rescue experiments
- SLCF-1::GFP localization data
- Identification of SLCF-1 substrates
- Data on SLCF-1 dynamic regulation from HEK293T transport assays and promotor constructs
- Assessment of mitochondrial morphology, activity, and stress
- Data on broader metabolic pathway alterations
Technical requirements
- Use of Caenorhabditis elegans as a genetic model
- Genetic screening techniques
- HEK293T transport assays
- Mutant slcf-1 promotor constructs
- Techniques for quantifying intestinal calcium waves (ICWs)
- Methods for assessing mitochondrial morphology, activity, and stress
