Summary
This NIH-funded research project (RP01) within a Program Project Grant (PPG) focuses on understanding the biochemical and structural mechanisms governing interactions between filoviruses (e.g., Ebola virus, Marburg virus) and their human hosts. The work aims to identify and validate molecular mechanisms at the host-viral interface using proteomic, biochemical, and advanced structural biology methods. Key goals include characterizing filoviral nucleocapsid interactions, mapping protein-protein interactions (PPIs) including post-translational modifications (PTMs) in infected cells, and defining molecular mechanisms contributing to filoviral infection to identify new antiviral targets.
What they want
Research Project 1 (RP01) will employ proteomic, biochemical, and hybrid structural methods — including mass spectrometry, NMR, X-ray crystallography, small angle X-ray scattering (SAXS), cryoelectron microscopy (cryo-EM), and cryoelectron tomography (cryo-TM) — to: (1) Determine the structural basis and dynamics of the filoviral nucleocapsid (NC) and define NC-host interactions; (2) Develop protein-protein interaction (PPI) maps that include post-translational modifications (PTMs) from virally infected cells for EBOV and MARV; and (3) Define the molecular mechanisms for PPIs that contribute to filoviral infection as defined by RP01, RP02, and RP03. The project operates collaboratively with other research projects (RP02, RP03) and scientific cores (Core B, Core C) within the broader PPG structure
