Summary
This research project aims to understand the roles of G-protein coupled receptors (GPCRs) during embryonic organ formation, focusing on germ cell navigation and salivary gland development in the Drosophila embryo.
GPCR signaling during embryonic organ formation
US · IL
NIH
grant
awarded
#nih-5R01GM145873-03
What they want
The project proposes to investigate the Drosophila GPCR Tre1, which is implicated in germ cell (GC) navigation and survival, immune cell extravasation, and neuroblast polarization. It will uncover the molecular and cellular mechanisms of the Tre1 pathway, from receptor binding to actin polymerization, and examine how other genes (Wunen, HMGCoA reductase) interact with this pathway. The research will also explore the function of Tre1 and Mthl5 in salivary gland (SG) navigation and establish a pipeline to screen all Drosophila embryonic GPCRs for their roles in GC or SG development.
Deliverables
- Uncover molecular and cellular mechanisms of the Tre1 pathway (receptor binding to actin polymerization)
- Determine if and how other genes affecting GC migration (Wunen, HMGCoA reductase) work through the Tre1 pathway
- Investigate if Hh signaling and Tre1 function in SG navigation
- Determine if Tre1 function in SG complements or antagonizes Mthl5 function
- Establish a pipeline to screen all Drosophila embryonic GPCRs for roles in GC or SG development
- Identify GPCRs with important functions in GC survival or SG extracellular matrix regulation
Technical requirements
- Experimental system of the Drosophila embryo
- Extensive armamentarium of genetic tools
Market context
inferred from NAICSR&D in Physical, Engineering, Life Sciences (except Nanotech & Biotech)
NAICS 541715
- US market size
- $95B
- Typical award
- $100K – $50M+
Typical buyers
DoDNSFNIHNASADOE
Commonly required
DCAA-compliant accountingITARCMMC L2
