Summary
This research project investigates the neuroprotective role of SULT4a1 in stroke by examining its impact on redox homeostasis and mitochondrial function, aiming to identify it as a potential therapeutic target.
SULT4a1, a novel neuroprotective protein in stroke
US · IL
NIH
grant
awarded
#nih-5R01NS119479-05
What they want
The project aims to study the role of SULT4a1 in stroke through four specific aims: 1) determine if SULT4a1 can maintain redox homeostasis via PRDXs in stroke, 2) assess if SULT4a1 can rescue mitochondrial function in stroke, 3) establish if SULT4a1 is neuroprotective in stroke, and 4) identify the mechanisms leading to the loss of SULT4a1 in stroke. The research will utilize oxygen-glucose deprivation in mouse cortical neurons and a middle cerebral artery occlusion (MCAO) model in mice.
Deliverables
- Essential knowledge about the role of novel SULT4a1-dependent pathways in stroke
- Determination of whether SULT4a1 can represent a credible target for therapeutic intervention in stroke
Technical requirements
- Oxygen-glucose deprivation in mouse cortical neurons
- Middle cerebral artery occlusion (MCAO) model of stroke in mice
- Viral-mediated expression of SULT4a1
- Seahorse Flux analysis
- Electron microscope imaging
- Proximity ligation assays
- Proximity-dependent biotin identification
- Live-cell imaging
- MRI imaging
