Development and testing of RSV vaccines using a computational framework of virus-host interaction

Summary

This project aims to develop and test polyvalent Respiratory Syncytial Virus (RSV) vaccine formulations using a computational framework and murine models to enhance antibody responses to conserved viral regions and improve protection against RSV disease.

What they want

The project involves three main aims: 1) Determining the effect of polyvalent RSV vaccine formulations on humoral immunity using computer simulations (ssMod.v2) to evaluate antibody specificity, cross-reactivity, and protection against RSV challenge. 2) Comparing the host immune response to polyvalent vaccine formulations in mice, utilizing mRNA-LNP vaccines encoding G or F from A2 or B1 RSV variants to induce specific antibodies and immune cells. 3) Testing if RSV polyvalent vaccine formulations improve protection from RSV disease in a murine model, assessing disease severity and neutralizing antibody titers using a primary human lung epithelial cell RSV-neutralization assay.

Deliverables

Computational evaluation of polyvalent vs. monovalent RSV vaccine formulations
Data on antibody specificity, cross-reactivity, and protection from computational framework
Constructed mRNA-LNP vaccines (encoding G or F from A2 or B1 RSV variants)
Murine immunization study results
Data on induced antibodies and immune cells in mice
Results from murine model of RSV challenge regarding disease severity
Neutralizing antibody titer data from human lung epithelial cell assay
Comparative analysis of infection and disease severity outcomes

Technical requirements

Computational framework of virus/host-interaction (ssMod.v2)
mRNA-LNP vaccine construction (cap-1, codon-optimized, structure-stabilized mRNA, ionizable cationic lipids)
Murine immunization protocols
Murine model of RSV challenge
Primary human lung epithelial cell RSV-neutralization assay

Market context

inferred from NAICS

Professional, Scientific & Technical Services

NAICS 541714

US market size: $2.0T
Typical award: $25K – $50M

Typical buyers

All federal civilianDoDStates

Commonly required

8(a)WOSBSDVOSBPE/PMP

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