Vesicle-mediated drug resistance of Candida albicans biofilm

The research builds on previous findings regarding EV delivery of matrix components and the impact of ESCRT pathway components and the antifungal turbinmicin on EV production. The current investigation hypothesizes that studying loss-of-function TF mutants and their effectors will identify regulatory networks for biofilm EV production and cargo packaging, and uncover roles for EV cargo in biofilm biology and drug resistance. This will involve testing EV biofilm hypotheses using three distinct functional TF mutant groups, turbinmicin as a pharmacologic tool, and EV and biofilm assays.