Summary
This research project aims to comprehensively quantify systemic metabolic fluxes and fuel use during cold exposure in mice to understand cold-induced thermogenesis (CIT) and its potential therapeutic application for obesity.
Comprehensive quantification of fuel use in cold-induced thermogenesis in vivo
US · IL
NIH
grant
open
#nih-5R01DK135958-03
What they want
The project proposes to carry out a comprehensive quantification of systemic metabolic fluxes during cold exposure in mice. This includes three main aims:
Aim 1: Comprehensive quantification of whole-body fuel turnover during CIT in mice.
Aim 2: Comprehensive quantification of fuel use in individual tissues during CIT in mice.
Aim 3: Test the role of carbohydrate flux in Brown Adipose Tissue (BAT) during CIT.
The studies are enabled by techniques such as steady-state infusions with non-perturbative amounts of heavy isotope-labeled fuels in live, awake, and ambulatory mice, and acute kinetic studies to estimate rates of tricarboxylic acid cycle turnover and VO2 in individual tissues. The underlying hypothesis is that CIT is largely fueled from fat stores but critically depends on anaplerotic carbohydrate sources from the liver to sustain fatty acid oxidation.
Deliverables
- Comprehensive quantification of whole-body fuel turnover during CIT in mice
- Comprehensive quantification of fuel use in individual tissues during CIT in mice
- Testing of the role of carbohydrate flux in BAT during CIT
Technical requirements
- Steady-state infusions with non-perturbative amounts of heavy isotope-labeled fuels in live, awake, and ambulatory mice
- Acute kinetic studies to allow quantitative estimates of rates of tricarboxylic acid cycle turnover and VO2 in individual tissues
Risks & flags
- This appears to be a summary of an already funded research project or grant application, not an open procurement opportunity. The language 'We propose here to carry out...' suggests a proposal, and 'NIH RePORTER' typically lists funded projects.
