Summary
This research project aims to precisely define the role of repressor E2F transcription factors (E2F4 and E2F5) in mammary gland development and function, with implications for understanding breast cancer.
Transcriptional regulation of mammary gland development
US · IL
Eunice Kennedy Shriver National Institute of Child Health and Human Development
grant
open
#nih-5R01HD104606-04
What they want
The long-term goal is to define the role of transcriptional regulation in mammary gland development and function, informing studies of breast cancer. The immediate objective is to precisely define the role of repressor E2Fs in the mammary gland, based on the hypothesis that they regulate key mammary gland developmental genes. Specific aims include: 1) Characterizing mammary gland phenotypes associated with mammary epithelial cell specific knockout of E2F5. 2) Generating ChIP-Seq data and integrating it with E2F5 induced gene expression data to determine E2F5 targets, filtered and prioritized using additional mammary development datasets. 3) Examining scRNAseq data in virgin glands lacking E2F3 or E2F5.
Deliverables
- Characterization of mammary gland phenotypes associated with mammary epithelial cell specific knockout of E2F5
- ChIP-Seq data
- Integrated E2F5 induced gene expression data
- Determination of E2F5 targets (filtered and prioritized)
- Examination of scRNAseq data in virgin glands lacking E2F3 or E2F5
- Understanding of how transcriptional repression regulates development of the mammary gland
- Establishment of a role for E2F5 in mammary gland development
Technical requirements
- Mammary epithelial cell specific knockout of E2F5
- ChIP-Seq data generation and integration
- Gene expression data analysis
- scRNAseq data examination
- Knockout mice (E2F5, E2F4, E2F3)
