Summary
Research on the p53 tumor suppressor protein, focusing on its post-translational modifications, interactions with transcriptional cofactors, and the identification of novel inhibitors for therapeutic targets in cancer.
Tumor Suppressor Protein, p53
US · IL
NIH
grant
open
#nih-1ZIABC005599-35
What they want
The research investigates the p53 tumor suppressor protein's role in cellular stress response, including identifying distinct interacting partners for its N-terminal transactivation domains (TAD1 and TAD2) using peptide pulldowns and mass spectrometry. It also explores the diverse C-terminal post-translational modifications of p53, specifically Lys382 methylation, and its association with colorectal cancer stem cells and macrophages in the tumor microenvironment. The project aims to identify novel inhibitors of SETD8, an enzyme involved in p53K382me1, using high-throughput assays. Additionally, the research examines the mechanism of action of small molecules, like NSC59984, that reactivate mutant p53 by covalently modifying specific cysteine residues.
