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Mapping dynamic functional networks across environments and backgrounds

US · IL NIH grant awarded #nih-5R01HG005853-14

Summary

This project proposes to continue systematic analysis of complex genetic interaction networks and phenotypes in yeast and human cells to understand gene function and identify disease gene modifiers.

What they want

The project will perform systematic phenotypic and genetic analyses in wild, genetically diverse yeast strains to identify genetic modifiers underlying background-specific gene essentiality and map genetic interactions. It will also produce quantitative cell biological phenotypic profiles associated with gene perturbation, exploring the influence of cell state on genetic perturbation effects using proteome dynamics. Finally, it aims to map a global genetic interaction network for a human cell line by screening an informative set of query gene mutants, with an emphasis on essential genes, to expand the scaffold genetic network and map networks underlying functionally representative protein complexes.
Deliverables
  • Identification of genetic modifiers underlying background-specific gene essentiality
  • Quantification of the effect of genetic background on genetic networks and genotype-phenotype relationship
  • Quantitative cell biological phenotypic profiles associated with gene perturbation
  • Methods to use the proteome as a read-out for genotype-phenotype analysis
  • A global genetic interaction network for a human cell line
  • Annotation of human gene function
  • Identification of conserved network properties for discovering disease gene modifiers, including those underlying cancer cell genetic dependencies
Technical requirements
  • Systematic phenotypic and genetic analyses
  • CRSPR-Cas9 platform
  • Quantitative single cell read-outs
  • Proteome dynamics as a phenotypic read-out

Market context

inferred from NAICS
Professional, Scientific & Technical Services
NAICS 541714
US market size
$2.0T
Typical award
$25K – $50M
Typical buyers
All federal civilianDoDStates
Commonly required
8(a)WOSBSDVOSBPE/PMP

Sector-level estimate — full code lookup not yet in catalog.

Mapping dynamic functional networks across…
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