Summary
This research project aims to elucidate the molecular and cellular mechanisms underlying peripheral cone survival in mouse models of Retinitis Pigmentosa (RP) to identify potential therapeutic targets.
Elucidating the molecular and cellular mechanisms underlying cone survival in the peripheral retina in mouse models of Retinitis Pigmentosa
US · IL
NIH
grant
awarded
#nih-5R00EY032110-05
What they want
The project involves a mentored (K99) phase and an independent (R00) phase. During the K99 phase, factors sufficient and/or necessary for cone survival will be identified using cell type specific RNA sequencing, in vivo retinal electroporation, and temporally-regulated gene deletion. The R00 phase will explore the causal relationship between blood-retina-barrier breakdown and cone degeneration.
Deliverables
- Identification of key regulators of cone survival in mouse models of RP
- Identification of a causal relationship between blood-retina-barrier breakdown and secondary cone death
Technical requirements
- Cell type specific RNA sequencing
- In vivo retinal electroporation
- Temporally-regulated gene deletion
Key personnel
- Researcher (grant recipient)
- Mentor (Dr. Constance Cepko for the mentored phase)
