Summary
Complex glycoconjugates play a pivotal role in bacterial survival, colonization, and virulence, and contribute to the interactions between symbiotic and pathogenic bacteria and their human hosts. Assembly of these macromolecules is initiated on the cytoplasmic face of cell membranes, catalyzed by polyprenol phosphate (PrenP) phosphoglycosyl transferases (PGTs). PGTs transfer a C1’-phosphosugar from a soluble nucleoside diphosphate-sugar to a PrenP acceptor, yielding a membrane-bound polyprenol diphosphosugar. Our studies focus on the exclusively prokaryotic PGT superfamily with a monotopic mem
