Core D: Digital Pathology and CyTOF

Summary

The Digital Pathology and Mass Cytometry Core (Core D) provides state-of-the-art technologies for high-parameter single-cell proteomic profiling to support research projects within a P01 Program Project Grant.

What they want

Core D offers established and validated assay platforms, including multicolor immune profiling (multispectral immunofluorescence staining) and high-dimensional proteomic profiling (Cytometry by Time-of-Flight; CyTOF). The core works with project investigators to assay human and mouse tissues, ensuring quality assurance and control for assays. Acquired data is then provided to Core C for analysis and integration, and to Core B for clinical correlations.

Deliverables

High-parameter single-cell proteomic profiling data
Multispectral immunofluorescence staining data
Mass cytometry (CyTOF) data

Technical requirements

Leica Bond automated immunostainers
Akoya automated high capacity multichannel high-resolution scanning microscopes (Vectra and Polaris systems)
Data servers linked by 10G and 40G internet connections
Helios™ third-generation cytometer

Key personnel

Core Co-Director (Dr. Anders, Associate Professor of Pathology, GI surgical pathologist)
Core Co-Director (Dr. Ho, Assistant Professor of Oncology, Scientific Director of CyTOF core)