Summary
This research project aims to identify the molecular mechanisms by which estrogens directly control osteoclast number and function, focusing on the antagonism of RANKL signaling, mitochondria activity, NAD levels, and apoptosis in osteoclasts.
What they want
The project will investigate the independent contributions and interactions of ECSIT, NAD, and apoptosis to osteoclastogenesis and the effects of estrogens. Experiments will involve using mice lacking ECSIT, Nampt, or Bak/Bax in osteoclast lineage cells, overexpressing mitochondria-targeted LbNOX in osteoclast cultures, and examining changes in osteoclast lineage cells in vivo and in vitro using single-cell RNA sequencing.
