Summary
This research project aims to define the cellular subpopulations of the Left-Right Organizer (LRO) and establish how they initiate left-right asymmetry in the developing embryo, focusing on the mouse LRO as a model for human LR development.
Left-Right Organizer Cellular and Molecular Dynamics Drive Early Cardiac Asymmetries
US · IL
NIH
grant
awarded
#nih-1F31HL179997-01
What they want
The project will investigate LRO structure and embryonic origins using single-cell and spatial transcriptomic data, followed by in vivo validation to identify and characterize LRO cell subpopulations. It will also investigate LRO function, expanding the model of LR symmetry breaking to include MMP21 mRNA and protein, using hybridization chain reaction in situ hybridization (HCR-FISH) and immunofluorescence (IF) experiments in mouse embryos with ciliary component and LRO gene mutations.
Technical requirements
- Mouse genetics
- Early vertebrate development
- scRNA-seq and spatial transcriptomic data generation and analysis
- Advanced microscopy image analysis
- Hybridization chain reaction in situ hybridization (HCR-FISH)
- Immunofluorescence (IF) experiments
