Summary
This project aims to develop novel single-cell and imaging mass cytometry methods using metal-bearing probes to analyze molecular pathways in aging systems, focusing on metabolic processes, autophagy, and senescence.
Single-Cell Molecular Pathway Analysis in Aging Systems via Novel Mass Cytometry Methods
US · IL
NIH
grant
awarded
#nih-5R01AG084804-03
What they want
The work involves developing single-cell and imaging methods with metal-bearing probes to monitor metabolic processes (e.g., siRNA delivery, protein synthesis, post-translational modification) in live cells. Specific aims include delivering molecular probes to viable cells for CyTOF analysis, barcoding prenylation probes for mixed single-cell samples, and exploring the effect of aging in murine primary cells by CyTOF and murine tissue by MIBI-ToF. The project will investigate the causal relationship between autophagy and senescence in murine liver models, specifically the link provided by the mevalonate pathway.
Deliverables
- Developed single-cell and imaging methods using metal-bearing probes for monitoring metabolic processes
- Expanded multi-parametric capabilities of mass cytometry (CyTOF, MIBI-ToF)
- Exploration of the causal relationship between autophagy and senescence in murine liver models
- Enhanced understanding of the relationship between autophagy, mevalonate, and senescence pathways in aging
- Information to inform emerging treatments to counteract detrimental effects of senescent cells
Technical requirements
- Mass cytometry (CyTOF)
- Multiplexed ion beam imaging by time-of-flight (MIBI-ToF)
- Metal-bearing probes
- Single-cell analysis
- Imaging methods
- siRNA delivery
- Protein synthesis monitoring
- Post-translational modification monitoring
- Barcoding prenylation probes
