Synthetic fentanyls adversely affect the blood-brain barrier and HIV replication in the context of neuroHIV

Summary

This research project investigates how synthetic fentanyls affect the blood-brain barrier (BBB) and HIV replication within the central nervous system (CNS) in the context of neuroHIV.

What they want

The study aims to examine the overall hypothesis that fentanyl and novel synthetic fentanyl analogs facilitate HIV-1 leukocyte transendothelial migration, microglial HIV infection/replication, and impair the integrity and function of the BBB. Aim 1 will use a tissue-engineered microfluidic model of the human BBB to analyze kinetic changes in BBB permeability, transporter status, and immune-endothelial interaction in response to fentanyl and other synthetic fentanyls. Aim 2 will determine the effects of synthetic fentanyls on dynamic HIV-1 infection in microglia, comparing them to monocyte-derived macrophages. Aim 3 will examine the molecular mechanisms responsible for fentanyl-induced dysfunctional BBB.

Deliverables

Important insights on how the interplay of HIV and fentanyl in the CNS impairs the BBB and compromises the intracellular anti-HIV immunity of microglia
Definition of underlying molecular mechanisms involved in fentanyl-induced BBB dysfunction

Technical requirements

Use of tissue-engineered microfluidic model of the human BBB
Experiments involving multiple synthetic fentanyls
Experiments to define underlying molecular mechanisms

Market context

inferred from NAICS

Professional, Scientific & Technical Services

NAICS 541714

US market size: $2.0T
Typical award: $25K – $50M

Typical buyers

All federal civilianDoDStates

Commonly required

8(a)WOSBSDVOSBPE/PMP

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