The Role of Neutrophils in Ischemia/Reperfusion Injury following Acute Stroke

Summary

Research into the mechanisms by which blocking neutrophil transendothelial migration (TEM) reduces stroke infarct size and its effect on stroke outcomes following ischemia/reperfusion injury.

What they want

The project aims to understand the mechanisms of myeloid cell TEM blockade in reducing stroke infarct size and the impact of interfering with neutrophil (PMN) extravasation on stroke outcomes. This involves two main aims: 1) identifying how TEM inhibition during ischemia/reperfusion injury alters the immune landscape of the stroke microenvironment, including leukocyte types and cytokine profiles; and 2) determining the therapeutic effect of blocking leukocyte extravasation versus selective PMN depletion following ischemia/reperfusion, assessing brain pathology and mouse motor function at various time points using TEM-blocking antibodies and PMN depletion.

Deliverables

Identification of differences in leukocyte types and cytokine profiles due to TEM blockade
Determination of the therapeutic effect of blocking leukocyte extravasation compared to selective PMN depletion
Insight into mechanisms regulating PMN response to I/R injury
Potential identification of a therapeutic intervention for stroke

Technical requirements

Analysis of leukocyte composition
Analysis of cytokine profiles
Studies conducted at different time points after reperfusion
Assessment of brain pathology
Assessment of mouse motor function
Use of TEM-blocking antibodies
Selective depletion of PMN