Summary
The Omics core (Core 2) will generate multiple layers of omics data from biospecimens collected from well-characterized cohorts (ADNI, MCSA, and 5 ADRCs) to identify molecular changes related to rich phenotypic data across diverse populations, supporting an overall NIH U19 proposal.
What they want
The Omics core will coordinate the collection, storage, and distribution of biospecimens for omics studies. It will generate high-quality omics data, harmonized across study sites, tissues, and cohorts, including bulk total RNA sequencing, single nucleus RNA sequencing (snRNASeq), proteome, metabolome, lipidome, DNA methylation, and genotype arrays. The core will also provide comprehensive biospecimen and omics documentation to facilitate data sharing within and outside the U19 program, accounting for and minimizing batch effects related to sample collection, processing, storage, and transfer.
Deliverables
- Harmonized omics data (RNAseq, snRNAseq, Proteome, Metabolome, Lipidome, DNA methylation array, genotype array)
- Comprehensive biospecimen and omics documentation
Technical requirements
- Omics measures: bulk total RNA sequencing, single nucleus RNA sequencing (snRNASeq), proteome, metabolome, lipidome, DNA methylation, genotype arrays
- Biospecimens: post-mortem brain tissue, ante-mortem blood, PAXgene blood, plasma, serum
- Cohorts: ADNI, MCSA, 5 ADRCs (Mayo Clinic, Indiana University, 1Florida, University of Michigan, Washington University)
- Populations: non-Hispanic white, Latino American, African American
- Methodology: Centralized coordination of biospecimen management, innovative study design, harmonized generation of multi-omics data, batch effect minimization
