Summary
This research project aims to elucidate the molecular mechanisms by which mediator proteins and Rad51 paralogs facilitate the formation of the pre-synaptic Rad51 nucleoprotein filament, a crucial step in homologous recombination DNA repair.
Pre-synaptic events in homologous recombination and maintenance of genomic stability
US · IL
NIH
grant
awarded
#nih-1R01CA305375-01
What they want
The project proposes to decipher how the ss-dsDNA junction is recognized through RPA-Rad52-Rad51 interactions, investigate the mechanisms by which Rad51 paralogs (Rad55-Rad57 and Csm2-Psy3-Shu1-Shu2) promote Rad51 filament formation, and establish how sorting and stacking properties are enacted in the human BRCA2 protein. The research will utilize ensemble and single-molecule Förster resonance energy transfer (FRET) fluorescence microscopy, structural mass-spectrometry, and C-trap analysis.
Technical requirements
- Ensemble and single-molecule Förster resonance energy transfer (FRET) fluorescence microscopy
- Structural mass-spectrometry
- C-trap analysis
