Summary
The Mutant Mouse Regional Resource Center (MMRRC) at the University of Missouri (MU) provides repository services for mutant mouse strains, cryopreserved germplasm, embryonic stem cells, and related reagents, while also conducting research to improve mouse model utility in biomedical research.
What they want
The MU MMRRC aims to continue and expand its services, including providing biomedical investigators with mouse models and related reagents, offering value-added services, and performing research broadly applicable to all mouse models. Over the next five years, the center will rederive imported mice to a pathogen-free state, cryopreserve gametes and embryos, perform genetic and infectious disease monitoring, and distribute live mice, cryopreserved germplasm, tissues, and embryonic stem cells. Research efforts will assess the role of complex gut microbiota in model phenotypes, reproducibility, and translatability by producing mice with defined humanized microbiota and developing tools for sampling microbiota kinetics in living mice. The MU MMRRC will also continue offering services such as cryopreservation, cryoresuscitation, rederivation, genotyping, assay development, genetic background complexity analysis, karyotyping, colony management, phenotyping, and microbiome manipulation and analysis.
Deliverables
- Live mice
- Cryopreserved germplasm
- Tissues
- Embryonic stem cells
- Mice harboring humanized complex microbiota
- Tools to better sample microbiota kinetics in living mice
Technical requirements
- Cryopreservation
- Cryoresuscitation from embryos or sperm
- Rederivation
- Genotyping and assay development
- Genetic background complexity analysis
- Karyotyping
- Colony management
- Phenotyping
- Microbiome manipulation and analysis
- Genetic monitoring
